{"product_id":"tropic-marin-professional-nitrite-nitrate-test-kit-freshwater-saltwater","title":"Tropic Marin Professional Nitrite\/Nitrate Test Kit - Freshwater\/Saltwater","description":"\u003cp\u003eNitrate ions (NO3–) are formed from ammonium (NH4+) as the end product of the nitrification process. Nitrite (NO2–), which is highly toxic to freshwater species in particular, is produced in the first stage of the bacterial process. If the nitrification process works, nitrite is converted to the comparatively non-toxic nitrate in the second stage. Increased levels of nitrite above 0.05 mg\/l (ppm) are usually found in tanks that are in their maturation phase or in tanks with nitrate filters.\u003c\/p\u003e\u003cp\u003eNitrate affects the quality of the water in the aquarium: Higher concentrations of nitrate in saltwater aquariums will end up inhibiting the growth of delicate coral. An algal bloom is often the result of a high nitrate level in an aquarium. Some reef aquariums have ultra-low nutrient conditions – nitrate shortages can occur in cases such as these. Therefore, the nitrate concentration in the aquarium water should be tested regularly.\u003c\/p\u003e\u003cp\u003eIt is advisable to keep the nitrate concentration in freshwater aquariums below 50 mg\/l (ppm), whereas the concentration should not exceed 20 mg\/l (ppm) in saltwater applications. When nurturing hard coral, the aim is to keep the nitrate concentration below 10 mg\/l (ppm). Lower limits for nitrate depend on the general conditions in the aquarium.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003ePackage Contents\u003c\/strong\u003e\u003c\/p\u003e\u003cul style=\"list-style-type: square;\"\u003e\n\u003cli\u003e50 ml\/1.69 fl.oz. of reagent A\u003c\/li\u003e\n\u003cli\u003e20 ml\/0.68 fl.oz. of reagent B\u003c\/li\u003e\n\u003cli\u003e10 ml\/0.34 fl.oz. of reagent C\u003c\/li\u003e\n\u003cli\u003e5 ml\/0.17 fl.oz. of reference solution “Standard”\u003c\/li\u003e\n\u003cli\u003e1 glass cuvette 20 ml\u003c\/li\u003e\n\u003cli\u003e1 dosing syringe 20 ml\u003c\/li\u003e\n\u003cli\u003e1 dosing syringe 1 ml with dropper tip\u003c\/li\u003e\n\u003cli\u003e2 colour cards\u003c\/li\u003e\n\u003cli\u003e1 comparator\u003c\/li\u003e\n\u003cli\u003e1 instructions for use\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eInstructions For Use\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eHigh concentrations:\u003c\/strong\u003e If the nitrate concentration is above 20 ppm (if the colours on the colour card are exceeded), dilute 3 ml of the test water with 12 ml of reverse osmosis water and carry outt he test again. The result is then multiplied by 5.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eDetermining the nitrite level:\u003c\/strong\u003e\u003c\/p\u003e\u003col\u003e\n\u003cli\u003eShake the dropper bottles before use!\u003c\/li\u003e\n\u003cli\u003eRinse out the glass cuvette with tap water and then several times with aquarium water.\u003c\/li\u003e\n\u003cli\u003eFill the glass cuvette with exactly 15 ml of aquarium water using the dosing syringe and place it in the comparator which serves as a cuvette stand.\u003c\/li\u003e\n\u003cli\u003ePlace the dropper tip on the 1 ml dosing syringe, add 1 ml test reagent A, close the glass cuvette using the stopper, briefly shake the solution and place it back in the comparator.\u003c\/li\u003e\n\u003cli\u003eThen add 4 drops of test reagent C, close the glass cuvette once again, shake it briefly and place it back in the comparator.\u003c\/li\u003e\n\u003cli\u003eAfter 3 minutes of development time, place the glass cuvette in the comparator on the white circles of the nitrite colour card in such a way that the second free opening on the comparator (without cuvette) is underneath the colour card (not on the colour fields) and the narrow end face of the comparator is facing the colour fields. Now the colour of the water sample is compared to the opposing colour fields by daylight conditions. To do this, look into the open cuvette from above. Move the sample on the colour card until the cuvette colour and the colour field colour match exactly.\u003c\/li\u003e\n\u003cli\u003eRead off the measured nitrite value under the relevant colour field. If the colours do not match exactly, an intermediate value can be estimated.\u003c\/li\u003e\n\u003c\/ol\u003e\u003cp\u003e\u003cstrong\u003eDetermining the nitrate level:\u003c\/strong\u003e\u003c\/p\u003e\u003col\u003e\n\u003cli\u003eShake the dropper bottles before use!\u003c\/li\u003e\n\u003cli\u003eRinse out the glass cuvette with tap water and then several times with aquarium water.\u003c\/li\u003e\n\u003cli\u003eFill the glass cuvette with exactly 15 ml of aquarium water using the dosing syringe and place it in the comparator which serves as a cuvette stand.\u003c\/li\u003e\n\u003cli\u003ePlace the dropper tip on the 1 ml dosing syringe, draw out 1 ml of test reagent A and add it to the water sample. Close the glass cuvette using the stopper, briefly shake the sample and place it back in the comparator.\u003c\/li\u003e\n\u003cli\u003eShake the bottle with test reagent B vigorously in a horizontal direction for approx. 30 seconds (the reagent must be well shaken). Then add 10 drops of test reagent B to the aquarium sample, close the cuvette again, shake it briefly and place it back in the comparator.\u003c\/li\u003e\n\u003cli\u003eAfter 3 minutes of development time, add 4 drops of test reagent C, close the glass cuvette once again, shake it briefly and place it back in the comparator.\u003c\/li\u003e\n\u003cli\u003eAfter another 3 minutes of development time, place the comparator on the white circles of the nitrate colour card in such a way that the second free opening on the comparator (without cuvette) is underneath the colour card (not on the colour fields) and the narrow end face of the comparator is facing the colour fields. Look into the open cuvette from above and compare it to the opposing colour fields by daylight conditions. Move the sample on the colour card until the cuvette colour and the colour field colour match exactly.\u003c\/li\u003e\n\u003cli\u003eRead off the measured nitrate value under the relevant colour field. Correct the result if necessary (see table below). If the colours do not match exactly, an intermediate value can be estimated.\u003c\/li\u003e\n\u003cli\u003eRinse out the glass cuvette and the syringe thoroughly with tap water after the measurement process.\u003c\/li\u003e\n\u003c\/ol\u003e\u003cp\u003e\u003cstrong\u003eUsing the “standard” to verify the test:\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003eThe shelf life of reagents depends on storage conditions and other factors. If the functional capacity of the test is inadequate, there will be no colour reaction during the test, even if nitrate levels are particularly high. If the measurement result is below 1 mg\/l (ppm), add five drops of the reference solution “standard” to a new sample in order to verify the reliability of the test. If the colour changes to pink (2 mg\/l (ppm)) when you carry out the test again, then the reliability of the reagents is verified as good.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eShelf life and storage: \u003c\/strong\u003e6 months after opening. Store in a cool, dark place.\u003c\/p\u003e","brand":"Tropic Marin","offers":[{"title":"Default Title","offer_id":57074498634111,"sku":"733019","price":25.49,"currency_code":"GBP","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0963\/5420\/6079\/files\/tropic_marin_nitrite_nitrate_professional_test.jpg?v=1772675552","url":"https:\/\/finestaquatics.co.uk\/products\/tropic-marin-professional-nitrite-nitrate-test-kit-freshwater-saltwater","provider":"Finest Aquatics","version":"1.0","type":"link"}